The České Budějovice branch has been serving the region since 2002. Its specialties include: water management structures, industrial buildings, health facilities, civic amenities, and residential developments. Reconstruction projects executed by the branch include the Train Station and Jirásek Weir in České Budějovice, the Hněvkovice Lock, the Třeboň Sauna World, and the old Eggenber Brewery building in Český Krumlov.

The Jihlava branch has been serving the Vysočina Region since 2010. It specializes in water management infrastructure, industrial buildings, civic amenities, and reinforced concrete applications. Notable current projects include the construction of new wards at the Pelhřimov Hospital, a new office building for the Jihlava Regional Authority, refrigeration plants in Unčovice, and a reinforced concrete frame for the ORGANICA Ostrava Project.

Division 9 has been operating in the Karlovy Vary Region since 2001. The unit builds and renovates sports facilities, shopping centers, rail structures, and much more. Past projects include reconstruction of the Goethe Lookout and the Emperor's Baths historical monument, enlargement of the Cheb Hospital, and construction of the Hazlov Complex and a residential building in Ostrov. 

More about Metrostav's operations in the Karlovy Vary Region.

The Pilsen regional branch was opened in 1994 to execute large residential, civil engineering, and transport infrastructure construction projects. In the region, the branch has completed such ventures as the Viktoria Stadium, Slovany Tramway Depot, the Faculty of Electrical Engineering of the University of West Bohemia, and the Green Triangle housing complex.

The Ústí Region branch was opened in 2008. It specializes in the construction of industrial facilities and water management structures. Projects executed by the branch include a new block of the Počerady Power Station, the Natural Science and Technology Center of the Purkyne University in Ústí nad Labem, the National Olympic Center for Water Sports in Račice, and pontoons on the Most, Milada, and Matylda Lakes. 

The Pardubice regional branch was opened in 2004. Its past projects in Pardubice include the reconstruction of the local hockey arena, swimming complex, and aqua park. Currently, the branch is renovating and enlarging the Tesco Shopping Center in Pardubice, reconstructing Winternitz Automatic Mills, and constructing new buildings for the Polytechnic Workshops and the Pardubice City Gallery.

The Liberec branch has been serving the region since 2000. The unit builds facilities for industry, transport, and civic amenities, such as schools and health facilities. The branch has over 35 clerical and technical employees who oversee all stages of the construction process. Past projects include a Bosch warehouse, the Lipová Multipurpose House in Liberec, the Emergency Medical Center in Jablonec nad Nisou, and the Česká Lípa Secondary and Vocational School. 

The Ostrava unit was established in 2004 to serve the North Moravian and Olomouc Regions. It specializes in structural engineering, both new construction and reconstruction, excavation work, and trenchless sewerage projects. The branch has set up a construction center that builds monolithic concrete structures. Notable past projects include the reconstruction of an indoor swimming pool, reconstruction of buildings in Karviná, revitalization of the Opava West Train Station, reconstruction of the Prasková Elementary School, and construction of the Silver Lake Recreation Resort. 

The Hradec Králové branch has been serving the region since 2004. The unit specializes in construction and reconstruction of buildings for clients from both the private and public sectors. Its current projects include reconstruction of the Faculty of Philosophy of the University of Hradec Králové and the renovation and enlargement of the Krkonoše National Park Visitor Center.